proteintech 11802 1 ap iab Search Results


proteintech 11802 1 ap iab  (Proteintech)
96
Proteintech proteintech 11802 1 ap iab
Proteintech 11802 1 Ap Iab, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proteintech 11802 1 ap iab/product/Proteintech
Average 96 stars, based on 1 article reviews
proteintech 11802 1 ap iab - by Bioz Stars, 2026-03
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tom20  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology tom20
TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate <t>TOM20.</t> Vinculin served as loading control.
Tom20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tom20/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
tom20 - by Bioz Stars, 2026-03
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anti-sirt7  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti-sirt7
TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate <t>TOM20.</t> Vinculin served as loading control.
Anti Sirt7, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-sirt7/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
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antibody anti-sox2  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology antibody anti-sox2
TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate <t>TOM20.</t> Vinculin served as loading control.
Antibody Anti Sox2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody anti-sox2/product/Santa Cruz Biotechnology
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antibody anti-sox2 - by Bioz Stars, 2026-03
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antibody anti-ho-1  (Proteintech)
90
Proteintech antibody anti-ho-1
TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate <t>TOM20.</t> Vinculin served as loading control.
Antibody Anti Ho 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody anti-ho-1/product/Proteintech
Average 90 stars, based on 1 article reviews
antibody anti-ho-1 - by Bioz Stars, 2026-03
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anti mouse igg  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc anti mouse igg
TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate <t>TOM20.</t> Vinculin served as loading control.
Anti Mouse Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mouse igg/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
anti mouse igg - by Bioz Stars, 2026-03
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tom20 11802-1-ap antibody  (Proteintech)
90
Proteintech tom20 11802-1-ap antibody
TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate <t>TOM20.</t> Vinculin served as loading control.
Tom20 11802 1 Ap Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tom20 11802-1-ap antibody/product/Proteintech
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tom20 11802-1-ap antibody - by Bioz Stars, 2026-03
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antitom20  (Proteintech)
96
Proteintech antitom20
TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate <t>TOM20.</t> Vinculin served as loading control.
Antitom20, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antitom20/product/Proteintech
Average 96 stars, based on 1 article reviews
antitom20 - by Bioz Stars, 2026-03
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tim23  (Proteintech)
96
Proteintech tim23
TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate <t>TOM20.</t> Vinculin served as loading control.
Tim23, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tim23/product/Proteintech
Average 96 stars, based on 1 article reviews
tim23 - by Bioz Stars, 2026-03
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antitomm20  (Proteintech)
95
Proteintech antitomm20
TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate <t>TOM20.</t> Vinculin served as loading control.
Antitomm20, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antitomm20/product/Proteintech
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antitomm20 - by Bioz Stars, 2026-03
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tom20 polyclonal antibody 11802-1  (Proteintech)
90
Proteintech tom20 polyclonal antibody 11802-1
TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate <t>TOM20.</t> Vinculin served as loading control.
Tom20 Polyclonal Antibody 11802 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tom20 polyclonal antibody 11802-1/product/Proteintech
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tom20 polyclonal antibody 11802-1 - by Bioz Stars, 2026-03
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membrane 20  (Proteintech)
96
Proteintech membrane 20
TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate <t>TOM20.</t> Vinculin served as loading control.
Membrane 20, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/membrane 20/product/Proteintech
Average 96 stars, based on 1 article reviews
membrane 20 - by Bioz Stars, 2026-03
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Image Search Results


TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate TOM20. Vinculin served as loading control.

Journal: bioRxiv

Article Title: Parkin-dependent ubiquitination of TAX1BP1 directs efficient autophagic removal of defective mitochondria

doi: 10.1101/2025.05.16.654474

Figure Lengend Snippet: TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate TOM20. Vinculin served as loading control.

Article Snippet: The following primary antibodies were used: Actin (1:50000, Sigma Aldrich, A5441), Calnexin (1:10000, Enzo Life Sciences, ADI-SPA-860), CS (1:6000, GeneTex, GTX110624), Flag-tag (1:10000, Sigma-Aldrich, F1804), LAMP1 (1:10000, DSHB Hybridoma bank, Clone H4A3), LC3B (1:10000, GeneTex, GT3612), LC3B (1:10000, Cell signaling, 835065), MFN1 (1:10000, Abnova, H00055669-M04), NBR1 (1:10000, ProteinTech, 16004-1-AP), NDP52 (1:10000, ProteinTech, 12229-1-AP), OPTN (1:10000, Atlas antibodies, HPA003360), SQSTM1/p62 (1:10000, BD Biosciences, 610832), Parkin (1:50000, Cell signaling, 4211S), TAX1BP1 (1:1000, Novus Biologicals, NBP1-86662), TOM20 (1:30000, Santa Cruz, sc-17764), TOM20 (1:6000, Proteintech, 11802-1-AP), VDAC (1:10000, ProteinTech, 66345-1), VDAC (1:30000, Millipore, AB10527), Vinculin (1:50000, Sigma Aldrich, V9131).

Techniques: Ubiquitin Proteomics, Transfection, Control

( A ) TAX1BP1 siRNA mediated knockdown in WT-Parkin HeLa cells does not impair VDAC and TOM20 degradation upon acute mitochondria depolarization. WT-Parkin HeLa cells were transfected with siRNA against TAX1BP1 for three consecutive days. After the third day, mitochondria were depolarized with CCCP for the indicated time points and protein quantification was performed. ( B ) WT-Parkin HeLa cells were transfected with His-tagged TAX1BP1 for 24h. Further, mitochondria were depolarized with 10 µm CCCP for the indicated time points. Left panel: Representative immunofluorescence images showing SSBP1 (cyan) and TAX1BP1 (yellow) upon mock or HIS-TAX1BP1 transfection after CCCP-mediated depolarization at the indicated time points. Right panel: Reduction of SSBP1 area after TAX1BP1 transfection after short (4h) or extended (14h) CCCP treatment. Scale bars: 5 µm. ( C ) Changes in ubiquitination pattern of His-tagged TAX1BP1 after 4h CCCP in 5KO-PARK HeLa cells.

Journal: bioRxiv

Article Title: Parkin-dependent ubiquitination of TAX1BP1 directs efficient autophagic removal of defective mitochondria

doi: 10.1101/2025.05.16.654474

Figure Lengend Snippet: ( A ) TAX1BP1 siRNA mediated knockdown in WT-Parkin HeLa cells does not impair VDAC and TOM20 degradation upon acute mitochondria depolarization. WT-Parkin HeLa cells were transfected with siRNA against TAX1BP1 for three consecutive days. After the third day, mitochondria were depolarized with CCCP for the indicated time points and protein quantification was performed. ( B ) WT-Parkin HeLa cells were transfected with His-tagged TAX1BP1 for 24h. Further, mitochondria were depolarized with 10 µm CCCP for the indicated time points. Left panel: Representative immunofluorescence images showing SSBP1 (cyan) and TAX1BP1 (yellow) upon mock or HIS-TAX1BP1 transfection after CCCP-mediated depolarization at the indicated time points. Right panel: Reduction of SSBP1 area after TAX1BP1 transfection after short (4h) or extended (14h) CCCP treatment. Scale bars: 5 µm. ( C ) Changes in ubiquitination pattern of His-tagged TAX1BP1 after 4h CCCP in 5KO-PARK HeLa cells.

Article Snippet: The following primary antibodies were used: Actin (1:50000, Sigma Aldrich, A5441), Calnexin (1:10000, Enzo Life Sciences, ADI-SPA-860), CS (1:6000, GeneTex, GTX110624), Flag-tag (1:10000, Sigma-Aldrich, F1804), LAMP1 (1:10000, DSHB Hybridoma bank, Clone H4A3), LC3B (1:10000, GeneTex, GT3612), LC3B (1:10000, Cell signaling, 835065), MFN1 (1:10000, Abnova, H00055669-M04), NBR1 (1:10000, ProteinTech, 16004-1-AP), NDP52 (1:10000, ProteinTech, 12229-1-AP), OPTN (1:10000, Atlas antibodies, HPA003360), SQSTM1/p62 (1:10000, BD Biosciences, 610832), Parkin (1:50000, Cell signaling, 4211S), TAX1BP1 (1:1000, Novus Biologicals, NBP1-86662), TOM20 (1:30000, Santa Cruz, sc-17764), TOM20 (1:6000, Proteintech, 11802-1-AP), VDAC (1:10000, ProteinTech, 66345-1), VDAC (1:30000, Millipore, AB10527), Vinculin (1:50000, Sigma Aldrich, V9131).

Techniques: Knockdown, Transfection, Immunofluorescence, Ubiquitin Proteomics

TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate TOM20. Vinculin served as loading control.

Journal: bioRxiv

Article Title: Parkin-dependent ubiquitination of TAX1BP1 directs efficient autophagic removal of defective mitochondria

doi: 10.1101/2025.05.16.654474

Figure Lengend Snippet: TAX1BP1 ubiquitination does not direct the autophagy adaptor to proteasomal degradation. WT-Parkin HeLa cells were transfected with HIS-tagged TAX1BP1 and subjected to mitochondrial depolarization for the indicated time points, in the presence (+) or absence (-) of the proteasomal inhibitior MG132. Mitophagy was confirmed by probing for the immediate-early protasome substrate MFN1 and the later autophagy substrate TOM20. Vinculin served as loading control.

Article Snippet: The following primary antibodies were used: Actin (1:50000, Sigma Aldrich, A5441), Calnexin (1:10000, Enzo Life Sciences, ADI-SPA-860), CS (1:6000, GeneTex, GTX110624), Flag-tag (1:10000, Sigma-Aldrich, F1804), LAMP1 (1:10000, DSHB Hybridoma bank, Clone H4A3), LC3B (1:10000, GeneTex, GT3612), LC3B (1:10000, Cell signaling, 835065), MFN1 (1:10000, Abnova, H00055669-M04), NBR1 (1:10000, ProteinTech, 16004-1-AP), NDP52 (1:10000, ProteinTech, 12229-1-AP), OPTN (1:10000, Atlas antibodies, HPA003360), SQSTM1/p62 (1:10000, BD Biosciences, 610832), Parkin (1:50000, Cell signaling, 4211S), TAX1BP1 (1:1000, Novus Biologicals, NBP1-86662), TOM20 (1:30000, Santa Cruz, sc-17764), TOM20 (1:6000, Proteintech, 11802-1-AP), VDAC (1:10000, ProteinTech, 66345-1), VDAC (1:30000, Millipore, AB10527), Vinculin (1:50000, Sigma Aldrich, V9131).

Techniques: Ubiquitin Proteomics, Transfection, Control

( A ) TAX1BP1 siRNA mediated knockdown in WT-Parkin HeLa cells does not impair VDAC and TOM20 degradation upon acute mitochondria depolarization. WT-Parkin HeLa cells were transfected with siRNA against TAX1BP1 for three consecutive days. After the third day, mitochondria were depolarized with CCCP for the indicated time points and protein quantification was performed. ( B ) WT-Parkin HeLa cells were transfected with His-tagged TAX1BP1 for 24h. Further, mitochondria were depolarized with 10 µm CCCP for the indicated time points. Left panel: Representative immunofluorescence images showing SSBP1 (cyan) and TAX1BP1 (yellow) upon mock or HIS-TAX1BP1 transfection after CCCP-mediated depolarization at the indicated time points. Right panel: Reduction of SSBP1 area after TAX1BP1 transfection after short (4h) or extended (14h) CCCP treatment. Scale bars: 5 µm. ( C ) Changes in ubiquitination pattern of His-tagged TAX1BP1 after 4h CCCP in 5KO-PARK HeLa cells.

Journal: bioRxiv

Article Title: Parkin-dependent ubiquitination of TAX1BP1 directs efficient autophagic removal of defective mitochondria

doi: 10.1101/2025.05.16.654474

Figure Lengend Snippet: ( A ) TAX1BP1 siRNA mediated knockdown in WT-Parkin HeLa cells does not impair VDAC and TOM20 degradation upon acute mitochondria depolarization. WT-Parkin HeLa cells were transfected with siRNA against TAX1BP1 for three consecutive days. After the third day, mitochondria were depolarized with CCCP for the indicated time points and protein quantification was performed. ( B ) WT-Parkin HeLa cells were transfected with His-tagged TAX1BP1 for 24h. Further, mitochondria were depolarized with 10 µm CCCP for the indicated time points. Left panel: Representative immunofluorescence images showing SSBP1 (cyan) and TAX1BP1 (yellow) upon mock or HIS-TAX1BP1 transfection after CCCP-mediated depolarization at the indicated time points. Right panel: Reduction of SSBP1 area after TAX1BP1 transfection after short (4h) or extended (14h) CCCP treatment. Scale bars: 5 µm. ( C ) Changes in ubiquitination pattern of His-tagged TAX1BP1 after 4h CCCP in 5KO-PARK HeLa cells.

Article Snippet: The following primary antibodies were used: Actin (1:50000, Sigma Aldrich, A5441), Calnexin (1:10000, Enzo Life Sciences, ADI-SPA-860), CS (1:6000, GeneTex, GTX110624), Flag-tag (1:10000, Sigma-Aldrich, F1804), LAMP1 (1:10000, DSHB Hybridoma bank, Clone H4A3), LC3B (1:10000, GeneTex, GT3612), LC3B (1:10000, Cell signaling, 835065), MFN1 (1:10000, Abnova, H00055669-M04), NBR1 (1:10000, ProteinTech, 16004-1-AP), NDP52 (1:10000, ProteinTech, 12229-1-AP), OPTN (1:10000, Atlas antibodies, HPA003360), SQSTM1/p62 (1:10000, BD Biosciences, 610832), Parkin (1:50000, Cell signaling, 4211S), TAX1BP1 (1:1000, Novus Biologicals, NBP1-86662), TOM20 (1:30000, Santa Cruz, sc-17764), TOM20 (1:6000, Proteintech, 11802-1-AP), VDAC (1:10000, ProteinTech, 66345-1), VDAC (1:30000, Millipore, AB10527), Vinculin (1:50000, Sigma Aldrich, V9131).

Techniques: Knockdown, Transfection, Immunofluorescence, Ubiquitin Proteomics